Development of PCR-based marker for resistance to Fusarium wilt race 2 in lettuce (Lactuca sativa L.)

Resistance to multiple races of Fusarium wilt is a desired trait in lettuce (Lactuca sativa L.) cultivars, as it directly related grower profitability. We focused on its resistance the race 2 pathogen, and analyzed F2 individuals obtained from cross between ‘VI185’ (resistance 2) ‘ShinanoGreen’ (susceptible for genetic mapping locus. According ddRAD-seq analysis genotype prediction each individual by infection assay derived F3 generation, single semi-dominant locus LG1 (qFOL1.2) was found determine resistance, marker designated LG1_v8_117.181Mbp showed complete co-segregation with phenotype. Fine identified PCR-based markers this allowed show that qFOL1.2 were located position 116.468–117.974Mbp LG1. Association phenotypes genotypes 42 cultivars all uniform susceptible while their resistant not always consistent. Nonetheless, only LG1_v8_116.506Mbp identical across 25 cultivars. Thus, we defined broadly available selection 2-resistance Our results provide additional breeding tool 2, can accelerate pyramiding loci program.